Fungi 73 100% satisfaction guaranteed Living Organisms valuable teacher resources: carolina.com Life Cycle Studies 155800P Pilobolus Kit (with perishables) (right) From 155806 Zygospore Demonstration Plate (Phycomyces blakesleeanus) Zygospore Demonstration Plates Show your students how conjugation fungi reproduce sexually by the fusion of gametes from 2 opposite mating types (+ and –) to form zygospores. Choose a plate culture showing the zygospores formed at the junction of the + and – mycelia of Phycomyces or Rhizopus. 155806 Phycomyces Each $27.65 155807 Rhizopus Each $28.10 Genetic Studies Carolina Teacher Tips QUESTION: How do I know when my Sordaria plate is ready for use? ANSWER: Use a wooden toothpick to scrape a few perithecia from the plate periphery, where the 2 strains have grown together. Place the perithecia on a microscope slide, add a drop of water, and gently press down on them with a coverslip. The perithecia should break open with gentle pressure and you should see asci with spores (they resemble bubblegum balls in a wrapper). You also should see a color difference between the 2 spore types in their crossover arrangement. Here are some signs the Sordaria asci are not ready to be used: • You must use a lot of pressure to break open the perithecia (the black spheres). • The spores are greenish in color. • You see only the dark, sac-like perithecia and no asci with spores. If the culture is not ready, continue to incubate the plate at 25° C (77° F) and repeat the testing process until the asci mature and rupture easily on the slide. If the culture is still not ready on Friday, the plate can be refrigerated over the weekend. Allow the plate and culture to come to room temperature before rechecking on Monday. Zygospore Kit Beginning—Easy to perform; requires no experience in microbiology. The Zygomycetes (or conjugation fungi) reproduce sexually by the fusion of gametes from 2 opposite mating types (+ and –) to form zygospores. Our zygospore kit is excellent for demonstrating both zygospores and the asexual stages of these fungi. A stereomicroscope is required. The exercise will take up to 7 days from setup to completion. With culturing instructions, 2 tube cultures of Rhizopus stolonifer (+ strain and – strain), life cycle illustration, 10 plates of appropriate mating agar, fixing solution, an inoculating needle, and an autoclave disposal bag. 155827 Per kit $40.50 Sordaria Demonstration Cross Plate Intermediate—Easy to perform; requires some basic training in microbiology. A cross of Sordaria fimicola wild type and the mutant tan strain for demonstrating genetic crossing over. For use with classic Advanced Placement® Biology Mitosis and Meiosis Lab or as a separate refresher demonstration from the exercises found in our Sordaria genetics kits (item #155847 and item #155848). Note: The culture is shipped on Fridays only and is ready to use the following Wednesday, Thursday, or Friday. Please allow 2 weeks’ advance notice with specific Friday shipping date. 155846 Per plate $29.00 Pilobolus Kit Beginning—Easy to perform; requires no experience in microbiology. The popular “shotgun” fungus, Pilobolus crystallinus, grows on herbivore dung. Herbivores will not graze near their own dung, so the spores must be propelled beyond this “zone of repugnance.” The sporangia bend toward the sun and “shoot” their spore mass in that direction. Students grow Pilobolus until sporangia are mature. Then they place the culture inside a container with a small hole to allow in light. The sporangia will all be propelled toward the hole. Requires about 10 days to conduct the exercise. The adaptation of this interesting fungus to its unique habitat is explained in the teacher’s instructions. Note: Order the kit with the perishable materials (all . items) included or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Keep fungus culture at room temperature; do not incubate or refrigerate. Keep agar plates refrigerated until use; do not freeze. Kit includes: Pilobolus crystallinus Plate Culture. 10 Plates Rabbit Dung Agar. Sterile Disposable Scalpel 10 Aluminum Foil Sheets 10 Containers with Lids Autoclave Disposal Bag Teacher Instructions 155800 Kit (with prepaid coupon) Each $51.40 155800P Kit (with perishables) Each $51.40 Advanced Placement® is a trademark registered and/or owned by the College Board®, which was not involved in the production of, and does not endorse, this product.
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