26 Viruses carolina.com Living Organisms ph: 800.334.5551 fax: 800.222.7112 Plant Viruses Tobacco Mosaic Virus Tobacco mosaic virus (TMV), representative of the tobamovirus group, affects nearly 200 known genera of plants and causes severe damage in tomato and tobacco crops. Because of the economic significance, TMV has been the subject of vigorous investigation. The physical and chemical nature and the penetration-replication infection cycle of the viral particle are well understood. TMV currently serves as an important research model in molecular biology and biotechnology. The infective TMV particle is a rigid rod, 300 nm L, consisting of single-stranded RNA wrapped within a protective coat of protein. The coating enables the virus to survive adverse environmental conditions. As such, TMV is very stable; frozen or lyophilized diseased plant tissue may remain infectious indefinitely. TMV poses no health hazard to students handling it in the laboratory. Tobacco Mosaic Virus Microscope Slide 126000 TMV Infectivity Assay Kit TMV Infectivity Assay Kit A safe introduction to virology for a class of 20 to 40 students. Students inoculate newly grown host plant primary leaves with tobacco mosaic virus (TMV). Necrotic local lesions that develop on the inoculated leaf indicate the infectivity of the virus and the susceptibility of the host. Students can also explore conditions that might affect infectivity/susceptibility by: (1) serially diluting the TMV inoculum; (2) using a buffer as diluent; and (3) heating the virus or host leaf. Kit includes all materials needed to conduct the exercises except water. With instructions. Note: Kit contains perishable material (. item). Kit and perishable material will ship separately and may arrive on different days. Keep sap frozen until use. Allow 9 to 10 days for assay plants to grow from seed; local disease symptoms appear on leaves 4 to 5 days after inoculation. Kit includes: Tobacco Mosaic Virus Frozen in Sap. TMV Buffer Solution 50 Foam Applicators Host Plant Seeds (pinto bean) 30 Plastic Vials 10 Straight Pins 30 Peat Pots 30 Dropping Pipets Bag Potting Soil 10 Vials Abrasive Powder 126000 Per kit $123.60 Tobacco Mosaic Virus, sec. 12-µm, sec., infected leaf including damaged and healthy areas. 293866 Each $6.85 12+ Each $6.60 Teacher Resources Virus Posters 18 × 24". These posters bring viruses up close and personal without the threat of contamination. Virus I shows the 3-D structure of these well-known viruses: adenovirus, the virus that causes foot-and-mouth disease; tobacco mosaic; influenza; HIV; smallpox; Ebola; and bacteriophage P22. Virus II shows 4 ways viruses enter animal cells and describes the step-by-step process of influenza virus replication. Virus III covers bacteriophages and follows the lytic and lysogenic cycles of replication. Phages shown on Virus III include filamentous phages Phi 29, P22, and T4. Laminated. 126200 Virus I Each $31.00 126201 Virus II Each $31.95 126202 Virus III Each $31.45 Carolina Teacher Tips Tips on Propagating Viruses • Propagating a virus is an easy, routine procedure if the host is susceptible to infection. • Select a host that is genetically susceptible, such as a strain of bacteria known to support the growth of the bacteriophage being studied. • Be sure the host cells have high metabolic activity so they are physiologically susceptible as well. • Bacteria in logarithmic growth, young insect larvae, and newly expanded primary leaves are excellent hosts for the appropriate viruses.
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