24 Viruses carolina.com Living Organisms ph: 800.334.5551 fax: 800.222.7112 121150 Basic Bacteriophage Culture Set A Basic Bacteriophage Culture Set A Recommended for introducing students to virulent bacterial viruses. Knowledge of techniques in handling bacteriophage is essential in modern genetics and genetic engineering. Set contains 5 mL each of high-titer phages T4 and T4r with one agar culture of E. coli B. Includes general information booklet. Note: Refrigerate coliphage and antiserum until use; do not freeze. Keep bacteria culture and agar at room temperature. 121150 Per set $47.00 121160 Basic Bacteriophage Culture Set B Basic Bacteriophage Culture Set B Recommended for introducing students to virulent bacterial viruses. Knowledge of techniques in handling bacteriophage is essential in modern genetics and genetic engineering. Set contains 5 mL each of high-titer phages T4r and FX174 with one agar culture each of E. coli B and E. coli C. Includes general information booklet. Note: Refrigerate coliphage and antiserum until use; do not freeze. Keep bacteria culture and agar at room temperature. 121160 Per set $60.60 High School & Introductory Sets Coliphage Stock Suspensions: 3 mL 1 × 108 T4r llA Known Mutant Tester. 5 mL 1 × 109 T4r ll Unknown Mutant No. 1. 5 mL 1 × 109 T4r ll Unknown Mutant No. 2. 5 mL 1 × 109 T4r ll Unknown Mutant No. 3. 3 mL 1 × 109 T4r llAB Reference Deletion Mutant X. 3 mL 1 × 109 T4r llAB Reference Deletion Mutant Y. 3 mL 1 × 109 T4r llAB Reference Deletion Mutant Z. Bacteria Stock Cultures: Escherichia coli B Agar Slant Culture. Escherichia coli K12 (lambda) Agar Slant Culture. Media and Glassware (sterile): Bottle Nutrient Peptone Broth (80 mL) 2 Bottles Tryptone Base Layer Agar 3 Tubes Nutrient Peptone Broth (5 mL) 4 Tubes Serial Dilution Tryptone Broth (9 mL) 13 Tubes Serial Dilution Tryptone Broth (9.9 mL) 6 Screw-Cap Vials (labeled for genetic crosses) 10 Tubes Tryptone Soft Agar Dropper Vial (5 mL) 35 Sterile Serological Pipets (1 mL) 3 Pasteur Pipets (9") 21 Glass Spotting Rods (5 × 150 mm) 20 Sterile Petri Dishes Other Supplies: 3 Bulbs for Pasteur Pipets Wax Marker 10 Flow Sheets 10 Manuals with Worksheets 124550 Kit (with prepaid coupon) Each $245.20 124550P Kit (with perishables) Each $245.20 A B C D Diagrammatic representation of bacteriophages (A) T2 intact (sheath extended), (B) T2 showing expulsion of DNA through the central core (sheath contracted), (C) lambda intact, and (D) FX174 intact, surface view, double scale; T2 and lambda are drawn to the scale indicated. 124550P Genetic Complementation and Recombination Spot Test Kit (with perishables) Genetic Complementation and Recombination Spot Test Kit All materials and instructions for (1) conducting complementation (cis-trans) spot tests to place unknown T4rll mutations in either cistron A or B, and for (2) conducting recombination spot tests to determine by deletion mapping the A-segments within which the rllA mutations occur. Exercises are recommended for an individual, a team of 2 or 3 students, or use as a demonstration. Other students, however, can participate when they are given sets of result plates for personal analysis and conclusions. Results are obtained from 4 spot tests and 2 control plates. During the course of the experimental procedure described, up to 8 additional sets of 6 plates each can be easily prepared for distribution to other students. Kit provides enough phage and bacteria for such duplication of results, but the required agar plates and soft agar must be purchased separately. Note: Please refer to Bacteriophage Media and item #124730 Plaque Assay Plating Set on page 25. Order the kit with the perishable materials (all . items) included or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Refrigerate coliphage until use; do not freeze. Keep bacteria culture and agar at room temperature.
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